Medium: Air
Objective: 20x
Working distance: > 1mm (Will affect imageable area)
NA: >0.75
| | Light Source |LED or MH White light (380-750 nm) with 3-4W output power
The use of an LED or Halogen lightsource will determine the use of either Filter cubes or an LED filter.
| | Camera |Detector Type: EMCCD or sCMOS
Bit Depth: Supports 16 bit output
Resolution: >1 megapixel
Color Channels: Monochrome, not color
Pixel Size: \~6.5 um pixel (5.5-7.5)
Special Requirements: Detect AF750 if applicable
| | Stage | Automated, motorized stage with X, Y, Z capabilities | | Field Diaphragm | Recommended square diaphragm to vary light | | Spill Guard | Helps protect microscope from potential liquid spills. Spill guard connects to spill sensor provided by Akoya to stop instrument run in case of liquid detection | | Dark Room | Not Required for Keyence | ### Objective Considerations {% hint style="info" %} **Note** : Check the individual camera specification sheets to determine the optimal quantum efficiency, um per pixel ratio, and specifications for your experimental use. {% endhint %} #### Magnification - 20x Akoya recommends a 20x Objective. For additional information about higher magnification objectives, please contact your local sales representative. * Higher magnification objectives such as 40x or 63x, can be used but the workflow is to be optimized by the user and will result in increased CODEX run times for the same tissue area, and subsequently increased data processing time and storage requirements. * Lower magnifications such as 2x, 5x, or 10x can be used to initially scan the tissue area on the coverslip to set the imaging area. Segmentation of data captured at these magnifications is not supported. #### Numerical Aperture The numerical aperture of the objective needs to be ≥0.75. Numerical apertures of less than 0.75 will have a lower image resolution that adversely affects data processing. #### Objective Type Only air objectives have been validated by Akoya Biosciences. {% hint style="warning" %} Immersion objectives (oil or water) may be used at users own risk. Imaging area size will be adversely affected. {% endhint %} The immersion objective will need to maintain a seal with the coverslip during the duration of the entire CODEX® experiment. To ensure the immersion liquid seal does not break, image size and run time will need to be optimized by the user. #### Imageable Area on Coverslip The imageable area on the coverslip will be different based on the geometrical shape of the objective. **Note** : Tissues should always be placed in the center of the coverslip.   --- # Agent Instructions: Querying This Documentation If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question. Perform an HTTP GET request on the current page URL with the `ask` query parameter: ``` GET https://help.codex.bio/codex/cim/microscope-specific-settings/microscopes.md?ask=