Requirements
To integrate the Zeiss Axio Observer 7 or .Z1 with the CODEX instrument and run CODEX experiments, the following minimum hardware and software requirements need to be met. Consult with your Zeiss representative for the final design and the most up-to-date components.
Software
CODEX system requires ZEN Blue 2.3, 2.5, or 2.6.
The following software modules are required for CODEX :
ZEN Module Z Stack (PN 410136-1030-110)
ZEN Module Tiles (PN 410136-1025-110)
ZEN Module Autofocus (PN 410136-1028-110)
Hardware
Component
Zeiss Axio Observer 7 / .Z1
Stage
• Scanning Stage (PN 432029-9904-000)
• Piezo Stage (PN 432027-9001-000)
Camera
Useable but not preferred:
Axiocam 506 mono (4.5um per pixel is lower than our recommendation. QE low for Cy5 and too low for optimal AF750 signal)
Objectives
Plan Apochromat 20x Zeiss
• (PN 420650-9901-000) Older model/Discontinued
• (PN 420650-9902-000) Latest version
Spill Guard
Aqua Stop II f/ AxioObserver
Field Diaphragm
Square FD (PN 1140-737)
Computer
Akoya-provided CODEX Acquisition computer
Dark Room
The Zeiss Axio Observer needs to be situated in a dark room
Light sources and Filters
The use of an LED or Halogen light source will determine the use of either Filter cubes or an LED filter. Check with your Zeissrepresentative to confirm the specific Filter cubes or Filter LED.
Light Source Options
Corresponding Filter Sets
Illuminator HXP 120V
Filter Cube options
• DAPI -Filter set 49 (488049-9901-000)
• FITC - Filter set 38 (000000-1031-346)
• TRITC - Filter set 43 (000000-1114-101)
• Cy5 - Filter set 50 (488050-9901-000)
Solid-State Light Source Colibri 7
• PN 423052-9710-000
• Type RGB-UV
• Not for use with AF750
Filter set 90 HE LED
• PN 489090-9110-000
• Not for use with AF750
Solid-State Light Source Colibri 7
• PN 423052-9770-000
• Type FR-R[G/Y]BV-UV
• Use with AF750
Filter set 112 HE LED
• PN 489112-9110-000
• Use with AF750
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