CODEX® Support
  • Welcome
  • CODEX® Instrument Manager (CIM)
    • Overview
    • User Instructions
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      • Maintenance
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      • Settings
    • Microscope Specific Settings
      • Microscope Requirements - Overview
      • Keyence BZ-X800 and BZ-X700
        • Requirements
        • BZ-X800 Image Acquisition Settings
          • Multi-Point (BZ-X800)
        • BZ-X700 Image Acquisition Settings
          • Multi-Point (BZ-X700)
        • Exposure steps
      • Leica DMi8
        • Image Acquisition Settings
      • Zeiss Axio Observer
        • Requirements
        • Image Acquisition Settings
      • Exposure Time Settings Considerations
      • Determination of Signal to Noise Ratio
    • Technical Notes
    • Installation
      • CIM Upgrades
  • CODEX® Processor
    • Overview
    • Installation
      • Install
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    • User Instructions
      • Quick Start
      • Processing Options
      • Segmentation Options
      • Image Analysis Report
    • Technical Notes
      • Expected Input
      • Expected Output
      • Cycle Alignment
      • Background Subtraction
      • Deconvolution
      • Extended Depth of Field
      • Shading Correction
      • Tile Registration
      • Segmentation & Spillover
      • t-SNE
      • FCS Format
    • Troubleshooting
  • CODEX® Multiplex Analysis Viewer (MAV)
    • Overview
    • Installation
      • System Requirements
      • CODEX® MAV - Download & Install
      • Release Notes
    • User Instructions
      • Quick Start
      • Open Experiment
      • Main Menu
      • Overlay Manager
      • Marker Picker
      • Region Image
      • History Panel
      • Save / Open Analysis
      • Clustering
      • Population Table
      • Cells
      • Marker Manager
      • Gating
      • Voronoi Diagram
      • Other Analysis Operations
    • Technical Notes
      • Algorithm Details
      • FCS Format
    • Public Datasets
    • Troubleshooting
  • Workflow Documents
    • CODEX and microscope setup
    • Reagents and staining
  • Appendix
    • End User License Agreement
    • Third-party Licenses
      • CIM
      • Processor
      • MAV
  • PhenoCycler Analysis
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  1. CODEX® Multiplex Analysis Viewer (MAV)
  2. User Instructions

Overlay Manager

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Last updated 5 years ago

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In this internal frame you are able to manage all overlays on region images. There are three types of overlay layers: Segmentation, Population and Voronoi.

You can change visibility of each layer by clicking on corresponding checkboxes.

Also you can set opacity of each layer by adjusting corresponding sliders.

All overlays are placed in specific order. You can change order of overlays be clicking on up/down arrow buttons.

Scale bar overlay

Tile mask overlay

This layer shows the tile numbers net on the region image.

Note: In different experiments tile ordering may be different.

Segmentation overlay

This layer shows the cell segmentation outlines from the current segmentation run. Outlines are randomly colored.

Population overlay

Voronoi overlay

Removed areas overlay

Heatmap

This layer shows the distance between two points on the .

This layer is connected to the Population Manager and it is disabled by default. It will become enabled after at least one population is created. See for more information.

This layer relates with Voronoi diagram and it is disabled by default. It will become enabled after Voronoi diagram is calculated. See for more information.

This layer shows all areas that were from the experiment.

This layer relates with Density heatmap and it is disabled by default. It will become enabled after Density heatmap is calculated. See for more information.

region image
Population table
Voronoi diagram
Density heatmap
Overlay Manager
Change overlay visibility
Change overlay opacity
Change overlays order
Scale bar overlay
Tile mask overlay
Segmentation overlay
Population overlay
Voronoi overlay
Removed areas overlay
Density heatmap overlay
removed