CODEX® Support
  • Welcome
  • CODEX® Instrument Manager (CIM)
    • Overview
    • User Instructions
      • Experiment
      • Maintenance
      • Transfer Data
      • Settings
    • Microscope Specific Settings
      • Microscope Requirements - Overview
      • Keyence BZ-X800 and BZ-X700
        • Requirements
        • BZ-X800 Image Acquisition Settings
          • Multi-Point (BZ-X800)
        • BZ-X700 Image Acquisition Settings
          • Multi-Point (BZ-X700)
        • Exposure steps
      • Leica DMi8
        • Image Acquisition Settings
      • Zeiss Axio Observer
        • Requirements
        • Image Acquisition Settings
      • Exposure Time Settings Considerations
      • Determination of Signal to Noise Ratio
    • Technical Notes
    • Installation
      • CIM Upgrades
  • CODEX® Processor
    • Overview
    • Installation
      • Install
      • Update
      • Version History
    • User Instructions
      • Quick Start
      • Processing Options
      • Segmentation Options
      • Image Analysis Report
    • Technical Notes
      • Expected Input
      • Expected Output
      • Cycle Alignment
      • Background Subtraction
      • Deconvolution
      • Extended Depth of Field
      • Shading Correction
      • Tile Registration
      • Segmentation & Spillover
      • t-SNE
      • FCS Format
    • Troubleshooting
  • CODEX® Multiplex Analysis Viewer (MAV)
    • Overview
    • Installation
      • System Requirements
      • CODEX® MAV - Download & Install
      • Release Notes
    • User Instructions
      • Quick Start
      • Open Experiment
      • Main Menu
      • Overlay Manager
      • Marker Picker
      • Region Image
      • History Panel
      • Save / Open Analysis
      • Clustering
      • Population Table
      • Cells
      • Marker Manager
      • Gating
      • Voronoi Diagram
      • Other Analysis Operations
    • Technical Notes
      • Algorithm Details
      • FCS Format
    • Public Datasets
    • Troubleshooting
  • Workflow Documents
    • CODEX and microscope setup
    • Reagents and staining
  • Appendix
    • End User License Agreement
    • Third-party Licenses
      • CIM
      • Processor
      • MAV
  • PhenoCycler Analysis
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  1. CODEX® Processor
  2. Technical Notes

FCS Format

To facilitate the use of outside analysis tools, processor outputs FCS files that Multiplex Analysis Viewer requires. Equivalent CSV files are written out in the same folder.

Header

Description

cell_id:cell_id

Cell ID unique to experiment

region:region

Region 1-index within the experiment

tile_num:tile_num

Tile 1-index within the corresponding region

x:x

Location of cell center, 0 to tileWidth * regionWidth

y:y

Location of cell center, 0 to tileHeight * regionHeight

z:z

Location of cell center, 0 to numZSlices

x_tile:x_tile

Location of cell center within the corresponding tile, 0 to tileWidth

y_tile:y_tile

Location of cell center within the corresponding tile, 0 to tileHeight

size:size

Number of voxels within the segmented cell

cyc001_ch001:<marker_name>

Integrated pixel intensity of all voxels within the segmented cell, can be compensated or uncompensated

...

...

tsne_x:tsne_x

t-SNE x coordinate unique to region

tsne_y:tsne_y

t-SNE y coordinate unique to region

homogeneity:homogeneity

A measure of expression homogeneity. sum(expression) / sqrt(sum(expression^2))

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Last updated 5 years ago

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