# Deconvolution ![(Left) Deconvolution. (Right) Without deconvolution](/files/-LwEzkumJPwruk1NCBKK) Microvolution is a 3D deconvolution implementation developed by Dr. Marc Bruce, Ph.D. of Stanford Manish Butte Lab and commercially licensed by Microvolution, LLC. This GPU-accelerated package uses Richardson-Lucy deconvolution algorithm with point-spread function (PSF) estimation optimized to best utilize GPU memory & processing architecture. This package requires a commercial license. Microvolution jar file contains a license manager that can generate a license (.lic) file from a key. The file must be present in the same folder as the processor for activation. [Bruce, M. A., & Butte, M. J. (2013). Real-time GPU-based 3D deconvolution. Optics express, 21(4), 4766-4773.](https://www.osapublishing.org/oe/abstract.cfm?uri=oe-21-4-4766) [Microvolution White Paper](https://www.intelligent-imaging.com/wp-content/uploads/2019/01/DDN-Microvolution-White-Paper-V1.pdf) --- # Agent Instructions: Querying This Documentation If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question. Perform an HTTP GET request on the current page URL with the `ask` query parameter: ``` GET https://help.codex.bio/codex/processor/technical-notes/deconvolution.md?ask= ``` The question should be specific, self-contained, and written in natural language. The response will contain a direct answer to the question and relevant excerpts and sources from the documentation. Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.