Processing Options

Last updated 5 years ago

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Processing options can be found in the main tab.

Name

Details

Background Subtraction

Enable auto-fluorescence subtraction using "blank" cycles

Deconvolution

Enable deconvolution which can reduce out of focus light

Extended Depth of Field

Enable collapse of a z-stack into a single best focus image

Shading Correction

Enable correction for optical shading or vignetting

Diagnostic output

Enable output of additional diagnostic images

t-SNE

Enable t-SNE calculation (may take 15% longer)

Name

Details

Reference Cycle

Reference marker to be used for cycle alignment

Reference Channel

Reference marker to be used for cycle alignment

3D Cycle Alignment

Disable to lock cycles in the z-axis during alignment

Blind Deconvolution

Unnecessary for typical 20x air imaging

Deconvolution Iterations

For advanced users; non-default values may degrade image quality

Deconvolution Model

For advanced users

Last updated 5 years ago

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