Processing Options
Last updated
Last updated
Processing options can be found in the main tab.
Name | Details |
Background Subtraction | Enable auto-fluorescence subtraction using "blank" cycles |
Deconvolution | Enable deconvolution which can reduce out of focus light |
Extended Depth of Field | Enable collapse of a z-stack into a single best focus image |
Shading Correction | Enable correction for optical shading or vignetting |
Diagnostic output | Enable output of additional diagnostic images |
t-SNE | Enable t-SNE calculation (may take 15% longer) |
Name | Details |
Reference Cycle | Reference marker to be used for cycle alignment |
Reference Channel | Reference marker to be used for cycle alignment |
3D Cycle Alignment | Disable to lock cycles in the z-axis during alignment |
Blind Deconvolution | Unnecessary for typical 20x air imaging |
Deconvolution Iterations | For advanced users; non-default values may degrade image quality |
Deconvolution Model | For advanced users |
