# Tile Registration Due to minute inaccuracies in camera positioning, image registration is required to determine the exact relative position of adjacent tiles. Microscopy Image Stitching Tool (MIST) library uses CUDA FFT phase correlation to find offsets with the highest correlation between overlapping areas. MIST was originally developed by Dr. Timothy Blattner, Ph.D. at National Institute of Standards and Technology (NIST) Information Technology Laboratory (ITL), and is essential in minimizing tile stitching errors. [Chalfoun, J., Majurski, M., Blattner, T., Bhadriraju, K., Keyrouz, W., Bajcsy, P., & Brady, M. (2017). MIST: accurate and scalable microscopy image stitching tool with stage modeling and error minimization. Scientific reports, 7(1), 4988.](https://www.nature.com/articles/s41598-017-04567-y#Sec2) [GitHub Link](https://github.com/usnistgov/MIST) --- # Agent Instructions: Querying This Documentation If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question. Perform an HTTP GET request on the current page URL with the `ask` query parameter: ``` GET https://help.codex.bio/codex/processor/technical-notes/tile-registration.md?ask= ``` The question should be specific, self-contained, and written in natural language. The response will contain a direct answer to the question and relevant excerpts and sources from the documentation. Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.