> For the complete documentation index, see [llms.txt](https://help.codex.bio/codex/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.codex.bio/codex/processor/technical-notes/tile-registration.md). # Tile Registration Due to minute inaccuracies in camera positioning, image registration is required to determine the exact relative position of adjacent tiles. Microscopy Image Stitching Tool (MIST) library uses CUDA FFT phase correlation to find offsets with the highest correlation between overlapping areas. MIST was originally developed by Dr. Timothy Blattner, Ph.D. at National Institute of Standards and Technology (NIST) Information Technology Laboratory (ITL), and is essential in minimizing tile stitching errors. [Chalfoun, J., Majurski, M., Blattner, T., Bhadriraju, K., Keyrouz, W., Bajcsy, P., & Brady, M. (2017). MIST: accurate and scalable microscopy image stitching tool with stage modeling and error minimization. Scientific reports, 7(1), 4988.](https://www.nature.com/articles/s41598-017-04567-y#Sec2) [GitHub Link](https://github.com/usnistgov/MIST) --- # Agent Instructions This documentation is published with GitBook. GitBook is the documentation platform designed so that both humans and AI agents can read, navigate, and reason over technical content effectively. Learn more at gitbook.com. ## Querying This Documentation If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question. Perform an HTTP GET request on the current page URL with the `ask` query parameter, and the optional `goal` query parameter: ``` GET https://help.codex.bio/codex/processor/technical-notes/tile-registration.md?ask=&goal= ``` `ask` is the immediate question: it should be specific, self-contained, and written in natural language. `goal` is optional and describes the broader end goal you are ultimately trying to accomplish on behalf of the user. GitBook uses it to tailor the answer towards what is most useful for that goal. The response will contain a direct answer to the question and relevant excerpts and sources from the documentation. Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.